Using the Light Scattering Component of Optical Intrinsic Signals to Visualize In Vivo Functional Structures of Neural Tissues

  • Uma Maheswari Rajagopalan
  • Kazushige Tsunoda
  • Manabu Tanifuji
Part of the METHODS IN MOLECULAR BIOLOGY™ book series (MIMB, volume 489)


Visualization of changes in reflected light from in vivo brain tissues reveals spatial patterns of neural activity. An important factor which influences the degree of light reflected includes the change in light scattering elicited by neural activation. Microstructures of neural tissues generally cause light scattering, and neural activities are associated with some changes in the microstructures. Here, we show that the optical properties unique to light scattering enable us to visualize spatial patterns of retinal activity non-invasively (FRG: functional retinography), and resolve functional structures in depth (fOCT: functional optical coherence tomography).

Key words

Intrinisic signal imaging OCT optical coherence tomography light scattering 


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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Uma Maheswari Rajagopalan
    • 1
  • Kazushige Tsunoda
    • 1
    • 2
  • Manabu Tanifuji
    • 1
  1. 1.Laboratory for Integrative Neural Systems, RIKEN Brain Science InstituteJapan
  2. 2.Laboratory of Visual Physiology, National Institute of Sensory OrgansJapan

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