Use of non-radioactive detection method for North- and South-Western Blot

  • Claudia Franke
  • Daniel Gräfe
  • Holger Bartsch
  • Michael BachmannEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 536)


Many proteins bind to nucleic acids. For the first characterization of novel proteins, a fast and simple technique for testing their nucleic acid binding capabilities is desirable. Here, we describe the use of a northwestern and southwestern blot protocol for the evaluation of the DNA and RNA binding abilities of a novel putative methyl transferase HSPC133 (METTL5).

Key words

DNA binding RNA binding Northwestern blot Southwestern blot 



Special thanks for excellent technical support to Martina Franke (Inst. Immunology, Carl-Gustav Carus TU-Dresden, Germany).


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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Claudia Franke
    • 1
  • Daniel Gräfe
    • 1
  • Holger Bartsch
    • 1
  • Michael Bachmann
    • 1
    Email author
  1. 1.Institute Immunology, MTZ, Carl Gustav Carus University TU Dresden, Medical FacultyDresdenGermany

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