Use of non-radioactive detection method for North- and South-Western Blot
Many proteins bind to nucleic acids. For the first characterization of novel proteins, a fast and simple technique for testing their nucleic acid binding capabilities is desirable. Here, we describe the use of a northwestern and southwestern blot protocol for the evaluation of the DNA and RNA binding abilities of a novel putative methyl transferase HSPC133 (METTL5).
Key wordsDNA binding RNA binding Northwestern blot Southwestern blot
Special thanks for excellent technical support to Martina Franke (Inst. Immunology, Carl-Gustav Carus TU-Dresden, Germany).