Introduction to Protein Blotting

  • Biji T. Kurien
  • R. Hal Scofield
Part of the Methods in Molecular Biology book series (MIMB, volume 536)


Protein blotting is a powerful and important procedure for the immunodetection of proteins following electrophoresis, particularly proteins that are of low abundance. Since the inception of the protocol for protein transfer from an electrophoresed gel to a membrane in 1979, protein blotting has evolved greatly. The scientific community is now confronted with a variety of ways and means to carry out this transfer.

Key words

Western blotting Sodium dodecyl sulfate polyacrylamide gel electrophoresis Nitrocellulose membrane Polyvinylidene difluoride membrane 


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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  1. 1.Oklahoma Medical Research FoundationOklahoma CityUSA

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