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Cationic electrophoresis and eastern blotting

  • Engelbert Buxbaum
Part of the Methods in Molecular Biology book series (MIMB, volume 536)

Summary

Denaturing, discontinuous electrophoresis in the presence of SDS has become a standard method for the protein scientist. However, there are situations where this method produces suboptimal results. In these cases, electrophoresis in the presence of positively charged detergents like cetyltrimethylammonium bromide may work considerably better. Methods for electrophoresis, staining, and blotting of such gels are presented.

Key words

Disk electrophoresis Cationic electrophoresis CTAB electrophoresis Blotting Eastern blotting Detergent CTAB 

Notes

Acknowledgements

This work was supported in part by Kuwait University grant MPB029.

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  1. 1.Department of BiochemistryRoss University School of MedicineRoseauWest Indies

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