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Origins of Protein Blotting

  • Harry TowbinEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 536)

Summary

The development of protein blotting in its early days is recounted as arising from the need to tackle a specific analytical problem. Combining diverse elements of common methods and simple lab equipment resulted in a procedure of general utility. The expansion of the idea of carrying out immunoassays on membranes as predecessors of microarrays is briefly touched upon.

Key words

Blotting Western History Protein array 

References

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    Rocco, R.M. (2006) Western blots [commentary]. In: Rocco, R.M., ed. Landmark Papers in Clinical Chemistry, Elsevier, Amsterdam, 381–383.Google Scholar
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    Towbin, H., Staehelin, T., and Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76, 4350–4354.PubMedCrossRefGoogle Scholar
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    Hawkes, R., Niday, E., and Gordon, J. (1982). A dot-immunobinding assay for monoclonal and other antibodies. Anal Biochem 119, 142–147.PubMedCrossRefGoogle Scholar
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    O’Neill, R.A., Bhamidipati, A., Bi X., Deb-Basu, D., Cahill, L., Ferrante, J., et al. (2006) Isoelectric focusing technology quantifies protein signaling in 25 cells. Proc Natl Acad Sci USA 103, 16153–16158.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  1. 1.Institute of Pharmaceutical Sciences, ETH Zurich, HCI H 430ZurichSwitzerland

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