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Reaping the Benefits of SAGE

  • Stephen J. Robinson
  • Justin D. Guenther
  • Christopher T. Lewis
  • Matthew G. Links
  • Isobel A.P. Parkin
Part of the Methods in Molecular Biology™ book series (MIMB, volume 406)

Summary

Serial analysis of gene expression (SAGE) is a powerful technique which yields a digital measure of gene expression through the sequencing of libraries of specific mRNA-derived fragments, namely SAGE tags. This chapter introduces the methods and software tools that are available for researchers to analyze gene expression through SAGE analysis. A detailed examination of SAGE analysis in Arabidopsis thaliana using the publicly available analysis tool, SaskSAGE, is provided. The use of this software allows the user to maximize the information gained from SAGE experiments in a model system with a fully sequenced genome.

Key Words

Serial analysis of gene expression expression profile novel transcripts Arabidopsis thaliana SaskSAGE 

Notes

Acknowledgments

The development of the SaskSAGE website was funded in part by the Genome Prairie project ‘Functional Genomics of Abiotic Stress.’ The authors would like to thank Dr Andrew Sharpe (AAFC, Saskatoon Research Centre) for critical reading of the chapter.

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Copyright information

© Humana Press Inc. 2007

Authors and Affiliations

  • Stephen J. Robinson
    • 1
  • Justin D. Guenther
    • 1
  • Christopher T. Lewis
    • 1
  • Matthew G. Links
    • 1
  • Isobel A.P. Parkin
    • 1
  1. 1.Agriculture and Agri-Food, Saskatoon Research CentreSaskatoonCanada

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