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MultiPLX

Automatic Grouping and Evaluation of PCR Primers
  • Lauris Kaplinski
  • Maido Remm
Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 402)

Summary

In this chapter, we describe MultiPLX—a tool for automatic grouping of PCR primers for multiplexed PCR. Both generic working principle and step-by-step practical procedures with examples are presented. MultiPLX performs grouping by calculating many important interaction levels between the different primer pairs and then distributes primer pairs to groups, so that the strength of unwanted interactions is kept below user-defined compatibility level. In addition, it can be used to select optimal primer pairs for multiplexing from list of candidates. MultiPLX can be downloaded from http://bioinfo.ut.ee/download/. Graphical Web-based interface to most functions of MultiPLX is available at http://bioinfo.ut.ee/multiplx/.

Key Words

PCR multiplex primer design 

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Copyright information

© Humana Press 2007

Authors and Affiliations

  • Lauris Kaplinski
    • 1
  • Maido Remm
    • 1
  1. 1.Department of BioinformaticsUniversity of TartuTartu

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