- 3.3k Downloads
In this chapter, we describe MultiPLX—a tool for automatic grouping of PCR primers for multiplexed PCR. Both generic working principle and step-by-step practical procedures with examples are presented. MultiPLX performs grouping by calculating many important interaction levels between the different primer pairs and then distributes primer pairs to groups, so that the strength of unwanted interactions is kept below user-defined compatibility level. In addition, it can be used to select optimal primer pairs for multiplexing from list of candidates. MultiPLX can be downloaded from http://bioinfo.ut.ee/download/. Graphical Web-based interface to most functions of MultiPLX is available at http://bioinfo.ut.ee/multiplx/.
Key WordsPCR multiplex primer design
- 3.von Ahsen, N., Wittwer, C.T. and Schutz, E. (2001) Oligonucleotide melting temperatures under PCR conditions: nearest-neighbor corrections for Mg2+, deoxynucleotide triphosphate, and dimetyl sulfoxide concentrations with comparison to alternative empirical formulas. Clinical Chemistry 47, 1956–61.Google Scholar
- 8.Kaderali, L. (2001) Selecting Target Specific Probes for DNA Arrays. Universität zu Köln, Köln.Google Scholar