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Meiosis pp 99-114 | Cite as

Visual Markers for Detecting Gene Conversion Directly in the Gametes of Arabidopsis thaliana

  • Luke E. Berchowitz
  • Gregory P. Copenhaver
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 557)

Abstract

Measuring meiotic gene conversion is important both because of its role in the fundamental mechanisms of meiotic recombination and because of its influence on linkage relationships and allelic diversity in the genome. Historically, gene conversion has been most thoroughly examined in fungal organisms through the use of tetrad analysis. Here we describe a method for using tetrad analysis in the model plant Arabidopsis thaliana to detect and quantify gene conversion events – a resource unavailable in most other higher eukaryotic model systems.

Key words

meiosis recombination crossing-over heteroduplex DNA mismatch repair 

Notes

Acknowledgments

We would like to thank Jeff Sekelsky (UNC at Chapel Hill) for critical reading of the manuscript. We would also like to thank NSF (MCB-0618691) and DOE (DE-FGO2-05ER15651) for financial support.

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Luke E. Berchowitz
    • 1
  • Gregory P. Copenhaver
    • 1
  1. 1.Department of Biology and The Carolina Center for Genome SciencesUniversity of North CarolinaChapel HillUSA

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