Meiosis pp 77-97

Part of the Methods in Molecular Biology book series (MIMB, volume 557)

Analysis of Meiotic Recombination in Caenorhabditis elegans

  • Kenneth J. Hillers
  • Anne M. Villeneuve
Protocol

Abstract

Caenorhabditis elegans is an important experimental organism for the study of recombination during meiosis. A variety of techniques have been developed for the measurement of meiotic recombination in C. elegans, ranging from traditional genetic measures to direct cytological determination of chiasma frequency. Here, we provide methods for some of the varied approaches used for the study of meiotic recombination in these tiny but powerful worms.

Key words

crossing over PCR recombination chiasmata snip-SNP bivalent univalent 

References

  1. 1.
    Keeney, S., Giroux, C. N., and Kleckner, N. (1997) Meiosis-specific DNA double-strand breaks are catalyzed by Spo11, a member of a widely conserved protein family. Cell 88, 375–84.PubMedCrossRefGoogle Scholar
  2. 2.
    Dernburg, A. F., McDonald, K., Moulder, G., Barstead, R., Dresser, M., and Villeneuve, A. M. (1998) Meiotic recombination in C. elegans initiates by a conserved mechanism and is dispensable for homologous chromosome synapsis. Cell 94, 387–98.PubMedCrossRefGoogle Scholar
  3. 3.
    Kim, J. S., and Rose, A. M. (1987) The effect of gamma radiation on recombination frequency in Caenorhabditis elegans. Genome 29, 457–62.PubMedCrossRefGoogle Scholar
  4. 4.
    Petronczki, M., Siomos, M. F., and Nasmyth, K. (2003) Un menage a quatre: the molecular biology of chromosome segregation in meiosis. Cell 112, 423–40.PubMedCrossRefGoogle Scholar
  5. 5.
    Villeneuve, A. M. (1994) A cis-acting locus that promotes crossing over between X chromosomes in Caenorhabditis elegans. Genetics 136, 887–902.PubMedGoogle Scholar
  6. 6.
    Stiernagle, T. (2006) WormBook, ed. the C. elegans Research Community, http://www.wormbook.org.
  7. 7.
    Davis, M. W., and Hammarlund, M. (2006) Single-nucleotide polymorphism mapping. Methods Mol. Biol. 351, 75–92.PubMedGoogle Scholar
  8. 8.
    Brenner, S. (1974) The genetics of Caenorhabditis elegans. Genetics 77, 71–94.PubMedGoogle Scholar
  9. 9.
    Wicks, S. R., Yeh, R. T., Gish, W. R., Waterston, R. H., and Plasterk, R. H. (2001) Rapid gene mapping in Caenorhabditis elegans using a high density polymorphism map. Nat. Genet. 28, 160–4.PubMedCrossRefGoogle Scholar
  10. 10.
    Hammarlund, M., Davis, M. W., Nguyen, H., Dayton, D., and Jorgensen, E. M. (2005) Heterozygous insertions alter crossover distribution but allow crossover interference in Caenorhabditis elegans. Genetics 171, 1047–56.PubMedCrossRefGoogle Scholar
  11. 11.
    Nabeshima, K., Villeneuve, A. M., and Hillers, K. J. (2004) Chromosome-wide regulation of meiotic crossover formation in Caenorhabditis elegans requires properly assembled chromosome axes. Genetics 168, 1275–92.PubMedCrossRefGoogle Scholar
  12. 12.
    Alpi, A., Pasierbek, P., Gartner, A., and Loidl, J. (2003) Genetic and cytological characterization of the recombination protein RAD-51 in Caenorhabditis elegans. Chromosoma 112, 6–16.PubMedCrossRefGoogle Scholar
  13. 13.
    Martin, J. S., Winkelmann, N., Petalcorin, M. I., McIlwraith, M. J., and Boulton, S. J. (2005) RAD-51-dependent and -independent roles of a Caenorhabditis elegans BRCA2-related protein during DNA double-strand break repair. Mol. Cell. Biol. 25, 3127–39.PubMedCrossRefGoogle Scholar
  14. 14.
    Rinaldo, C., Bazzicalupo, P., Ederle, S., Hilliard, M., and La Volpe, A. (2002) Roles for Caenorhabditis elegans rad-51 in meiosis and in resistance to ionizing radiation during development. Genetics 160, 471–9.PubMedGoogle Scholar
  15. 15.
    Liu, H., Jang, J. K., Kato, N., and McKim, K. S. (2002) mei-P22 encodes a chromosome-associated protein required for the initiation of meiotic recombination in Drosophila melanogaster. Genetics 162, 245–58.PubMedGoogle Scholar
  16. 16.
    Thorne, L. W., and Byers, B. (1993) Stage-specific effects of X-irradiation on yeast meiosis. Genetics 134, 29–42.PubMedGoogle Scholar
  17. 17.
    Chin, G. M., and Villeneuve, A. M. (2001) C. elegans mre-11 is required for meiotic recombination and DNA repair but is dispensable for the meiotic G(2) DNA damage checkpoint. Genes Dev. 15, 522–34.PubMedCrossRefGoogle Scholar
  18. 18.
    Colaiacovo, M. P., MacQueen, A. J., Martinez-Perez, E., McDonald, K., Adamo, A., La Volpe, A., and Villeneuve, A. M. (2003) Synaptonemal complex assembly in C. elegans is dispensable for loading strand-exchange proteins but critical for proper completion of recombination. Dev. Cell 5, 463–74.PubMedCrossRefGoogle Scholar
  19. 19.
    Hayashi, M., Chin, G. M., and Villeneuve, A. M. (2007) C. elegans germ cells switch between distinct modes of double-strand break repair during meiotic prophase progression. PLoS Genet. 3, 2068–84.CrossRefGoogle Scholar
  20. 20.
    McKim, K. S., Howell, A. M., and Rose, A. M. (1988) The effects of translocations on recombination frequency in Caenorhabditis elegans. Genetics 120, 987–1001.PubMedGoogle Scholar
  21. 21.
    Edgley, M. L., Baillie, D. L., Riddle, D. L., and Rose, A. M. (2006) Genetic balancers. WormBook, ed. the C. elegans Research Community, http://www.wormbook.org.
  22. 22.
    Kelly, K. O., Dernburg, A. F., Stanfield, G. M., and Villeneuve, A. M. (2000) Caenorhabditis elegans msh-5 is required for both normal and radiation- induced meiotic crossing over but not for completion of meiosis. Genetics 156, 617–30.PubMedGoogle Scholar
  23. 23.
    Zetka, M. C., and Rose, A. M. (1990) Sex-related differences in crossing over in Caenorhabditis elegans. Genetics 126, 355–63.PubMedGoogle Scholar
  24. 24.
    Fire, A., Xu, S., Montgomery, M. K., Kostas, S. A., Driver, S. E., and Mello, C. C. (1998) Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391, 806–11.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Kenneth J. Hillers
    • 1
  • Anne M. Villeneuve
    • 2
  1. 1.Biological SciencesCalifornia Polytechnic State UniversitySan Luis ObispoUSA
  2. 2.Department of Developmental BiologyStanford University School of MedicineStanfordUSA

Personalised recommendations