Meiosis pp 65-76 | Cite as

Genetic Analysis of Meiotic Recombination in Schizosaccharomyces pombe

  • Gerald R. Smith
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 557)

Abstract

The fission yeast Schizosaccharomyces pombe is well-suited for studying meiotic recombination. Methods are described here for culturing S. pombe and for genetic assays of intragenic recombination (gene conversion), intergenic recombination (crossing-over), and spore viability. Both random spore and tetrad analyses are described.

Key words

fission yeast Schizosaccharomyces pombe meiosis intragenic recombination (gene conversion) intergenic recombination (crossing-over) spore viability tetrad analysis 

Notes

Acknowledgments

I am grateful to Sue Amundsen, Gareth Cromie, and Luther Davis for helpful comments on the manuscript. My laboratory is supported by research grants GM031693 and GM032194 from the National Institutes of Health.

References

  1. 1.
    Davis, L., and Smith, G.R. (2003) Non-random homolog segregation at meiosis I in Schizosaccharomyces pombe mutants lacking recombination. Genetics 163, 857–874.PubMedGoogle Scholar
  2. 2.
    Cromie, G.A., and Smith, G.R. (2008) Meiotic recombination in Schizosaccharomyces pombe: A paradigm for genetic and molecular analysis, in “Genome Dynamics and Stability” (Egel, R. and Lankenau, D-H., eds.), Springer-Verlag, Berlin, pp. 195–230.Google Scholar
  3. 3.
    Wood, V., Gwilliam, R., Rajandream, M.-A., Lyne, M., Lyne, R., Stewart, A., Sgouros, J., Peat, N., Hayles, J., Baker, S., Basham, D., et al. (2002) The genome sequence of Schizosaccharomyces pombe. Nature 415, 871–880.Google Scholar
  4. 4.
    Moreno, S., Klar, A.J.S., and Nurse, P. (1991) Molecular genetic analysis of fission yeast Schizosaccharomyces pombe. Methods Enzymol. 194, 795–823.Google Scholar
  5. 5.
    Egel, R. (Ed.) (2004) The Molecular Biology of Schizosaccharomyces pombe. Springer-Verlag, Berlin.Google Scholar
  6. 6.
    Iino, Y., and Yamamoto, M. (1985) Mutants of Schizosaccharomyces pombe which sporulate in the haploid state. Mol. Gen. Genet. 198, 416–421.CrossRefGoogle Scholar
  7. 7.
    Yamamoto, A., and Hiraoka, Y. (2003) Monopolar spindle attachment of sister chromatids is ensured by two distinct mechanisms at the first meiotic division in fission yeast. EMBO J. 22, 2284–2296.PubMedCrossRefGoogle Scholar
  8. 8.
    Tange, Y., Horio, T., Shimanuki, M., Ding, D.Q., Hiraoka, Y., and Niwa, O. (1998) A novel fission yeast gene, tht1 +, is required for the fusion of nuclear envelopes during karyogamy. J. Cell. Biol. 140, 247–258.PubMedCrossRefGoogle Scholar
  9. 9.
    Martín-Castellanos, C., Blanco, M., Rozalén, A.E., Pérez-Hidalgo, L., García, A.I., Conde, F., Mata, J., Ellermeier, C., Davis, L., San-Segundo, P., Smith, G.R., and Moreno, S. (2005) A large-scale screen in S. pombe identifies seven novel genes required for critical meiotic events. Curr. Genet. 15, 2056–2062.Google Scholar
  10. 10.
    Li, Y.F., and Smith, G.R. (1997) The Schizosaccharomyces pombe rec16 gene product regulates multiple meiotic events. Genetics 146, 57–67.PubMedGoogle Scholar
  11. 11.
    Niwa, O., and Yanagida, M. (1985) Triploid meiosis and aneuploidy in Schizosaccharomyces pombe: an unstable aneuploid disomic for chromosome III. Curr. Genet. 9, 463–470.CrossRefGoogle Scholar
  12. 12.
    Young, J.A., Schreckhise, R.W., Steiner, W.W., and Smith, G.R. (2002) Meiotic recombination remote from prominent DNA break sites in S. pombe. Mol. Cell 9, 253–263.PubMedCrossRefGoogle Scholar
  13. 13.
    Haldane, J.B.S. (1919) The combination of linkage values, and the calculation of distances between the loci of linked factors. J. Genet. 8, 299–309.CrossRefGoogle Scholar
  14. 14.
    Perkins, D.D. (1949) Biochemical mutants in the smut fungus Ustilago maydis. Genetics 34, 607–626.Google Scholar
  15. 15.
    Munz, P. (1994) An analysis of interference in the fission yeast Schizosaccharomyces pombe. Genetics 137, 701–707.PubMedGoogle Scholar

Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Gerald R. Smith
    • 1
  1. 1.Division of Basic Sciences, Fred Hutchinson Cancer Research CenterSeattleUSA

Personalised recommendations