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Meiosis pp 27-33 | Cite as

Modulating and Targeting Meiotic Double-Strand Breaks in Saccharomyces cerevisiae

  • Alain Nicolas
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 557)

Abstract

Meiotic recombination is initiated by DNA double-strand breaks (DSBs) formed by the evolutionary conserved Spo11 protein. Along the S. cerevisiae chromosomes, the DSB sites are not evenly distributed and the cleavage frequencies vary 10–100-fold from site to site. Herein are reviewed the methods used in budding yeast to modulate locally and globally the native DSB frequencies, including a powerful method to target Spo11-dependent meiotic DSB in novel chromosomal regions. These methods serve to investigate the control and the mechanism of recombination initiation and modify the natural distribution of meiotic recombination.

Key words

meiosis recombination initiation Spo11 

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Alain Nicolas
    • 1
  1. 1.Institut Curie, Centre de Recherche, UMR7147-CNRS, Université Pierre et Marie CurieParisFrance

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