Auditory and Vestibular Research pp 299-310 | Cite as
In Vivo Verification of Protein Interactions in the Inner Ear by Coimmunoprecipitation
Protocol
Genomics has provided us with vast amounts of data and thus, the challenge to identify and characterize gene products. Proteomics analysis, using methods such as yeast two-hybrid screenings, isoelectric focusing, and mass spectroscopy, generate potentially useful information. To determine functional relationships between and among proteins, however, the initial data for putative protein interactions must first be validated. One technique, which is considered the gold standard, is coimmunoprecipitation.
Inner ear ion channels protein-protein interactions coimmunoprecipitation
Notes
Acknowledgments
This work was supported by NIDCD grant DC04295 to B. H. A. S.
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