The MIRA Method for DNA Methylation Analysis
DNA methylation patterns are often altered in human cancer and aberrant methylation is considered a hallmark of malignant transformation. Several methods have been developed for the characterization of gene-specific and genome-wide DNA methylation patterns. In this chapter, we describe the methylated-CpG island recovery assay (MIRA), which is based on the high affinity of the MBD2b/MBD3L1 complex for double-stranded CpG-methylated DNA. MIRA has been used in combination with microarray platforms to map DNA methylation patterns across the human genome.
KeywordsDNA methylation analysis methyl-CpG binding protein microarrays CpG islands tiling arrays
This work was supported by NIH grants CA104967 and CA128495 to GPP.