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The MIRA Method for DNA Methylation Analysis

  • Tibor A. Rauch
  • Gerd P. Pfeifer
Part of the Methods in Molecular Biology book series (MIMB, volume 507)

Abstract

DNA methylation patterns are often altered in human cancer and aberrant methylation is considered a hallmark of malignant transformation. Several methods have been developed for the characterization of gene-specific and genome-wide DNA methylation patterns. In this chapter, we describe the methylated-CpG island recovery assay (MIRA), which is based on the high affinity of the MBD2b/MBD3L1 complex for double-stranded CpG-methylated DNA. MIRA has been used in combination with microarray platforms to map DNA methylation patterns across the human genome.

Keywords

DNA methylation analysis methyl-CpG binding protein microarrays CpG islands tiling arrays 

Notes

Acknowledgements

This work was supported by NIH grants CA104967 and CA128495 to GPP.

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Tibor A. Rauch
    • 1
  • Gerd P. Pfeifer
    • 2
  1. 1.Division of BiologyBeckman Research Institute of the City of HopeDuarteUSA
  2. 2.School of Engineering and ScienceJacobs University BremenGermany

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