Methylated DNA Immunoprecipitation (MeDIP)

  • Fabio Mohn
  • Michael Weber
  • Dirk Schübeler
  • Tim-Christoph Roloff
Part of the Methods in Molecular Biology book series (MIMB, volume 507)


Methylated DNA immunoprecipitation (MeDIP) is a versatile immunocapturing approach for unbiased detection of methylated DNA. In brief, genomic DNA is randomly sheared by sonication and immunoprecipitated with a monoclonal antibody that specifically recognizes 5-methylcytidine. The resulting enrichment of methylated DNA in the immunoprecipitated fraction can be determined by PCR to assess the methylation state of individual regions. Alternatively, MeDIP can be combined with large-scale analysis using microarrays as a genome-wide experimental readout. This protocol has been applied to generate comprehensive DNA methylation profiles on a genome-wide scale in mammals and plants, and further to identify abnormally methylated genes in cancer cells.


MeDIP methylated DNA DNA methylation genome-wide analysis epigenomics 5mC antibody CpG immunoprecipitation 



The authors would like to thank the members of the Schübeler lab for helpful comments on the protocol.


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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Fabio Mohn
    • 1
  • Michael Weber
    • 1
  • Dirk Schübeler
    • 1
  • Tim-Christoph Roloff
    • 1
  1. 1.Friedrich Miescher Institute for Biomedical ResearchSwitzerland

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