Restriction Digestion and Real-Time PCR (qAMP)
DNA methylation in mammals has been shown to play many important roles in diverse biological phenomena. Here we describe a simple and straightforward method that quantitatively measures site-specific levels of DNA methylation in a quick and cost-effective manner. The quantitative analysis of DNA methylation using real-time PCR (qAMP) technique involves the digestion of genomic DNA using methylation-sensitive and methylation-dependent restriction enzymes followed by real-time PCR. This approach generates accurate and reproducible results without the requirement for prior treatment of the DNA with sodium bisulfite.
KeywordsDNA methylation analysis method restriction enzyme real-time PCR
- 4.Clark, S. J., Harrison, J., Paul, C. L., et al. (1984) High sensitivity mapping of methylated cytosines. Nucleic Acids Res 22, 2990–2997.Google Scholar