Mitochondrial DNA pp 87-101

Part of the Methods in Molecular Biology™ book series (MIMB, volume 554) | Cite as

Methods for Assessing Binding of Mitochondrial Transcription Factor A (TFAM) to DNA

  • Atsushi Fukuoh
  • Dongchon Kang

Abstract

It is now recognized that mammalian mitochondrial DNA forms a higher structure called the nucleoid, corresponding to the nucleosome of nuclear DNA. Mitochondrial transcription factor A (TFAM), which was cloned as a transcription factor for mitochondrial DNA, is essential for the maintenance of mitochondrial DNA. In fact, TFAM markedly enhances the promoter-specific transcription of mitochondrial DNA. In addition, TFAM has an ability to bind to DNA in a sequence-independent manner and is abundant enough to cover an entire region of mitochondrial DNA. Over-expression of human TFAM in cells increases the amount of mitochondrial DNA almost in parallel with the TFAM. TFAM may stabilize mitochondrial DNA by packaging and regulate (or titrate) the amount of mitochondrial DNA. Thus, TFAM may play a crucial role in maintaining mitochondrial DNA as a main component of the nucleoid (or more appropriately mitochromosome).

Key words

Transcription mtDNA TFAM transcription factor nucleoid mitochromosome 

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Atsushi Fukuoh
    • 1
  • Dongchon Kang
    • 1
  1. 1.Department of Clinical Chemistry and Laboratory MedicineKyushu University Graduate School of Medical SciencesFukuokaJapan

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