Comparative Genomics-Based Orthologous Promoter Analysis Using the DoOP Database and the DoOPSearch Web Tool

  • Endre Barta
Part of the Methods in Molecular Biology™ book series (MIMB, volume 395)

Summary

Bioinformatic and experimental analyses of promoter regions are available for a long time. Finding of the transcription factor binding sites (TFBSs), however, by either method still faces a number of problems. For example, because of the ambiguity of binding of transcription factors, the number of false-positives and -negatives can be unexpectedly high in these sequence analyses. We can assume that evolutionary conserved motifs or regions in the promoters of the homologous genes function as TFBSs. Thus, a comparative genomic approach can provide a partial resolution for the problem previously outlined.

This chapter describes application of the DoOP database and the DoOPSearch web tools for such a comparative genomic analysis. Orthologous promoter sequences and conserved motifs can be extracted from the DoOP database for further analysis. The web-based tools of the DoOPSearch webpage can be used for searching and comparing conserved motifs. Using these tools, it is possible to compare short sequences with conserved motifs, to map conserved motifs into a longer promoter region, or find sequence patterns in different sets of promoter sequences.

Keywords

Promoter sequences orthologous promoters conserved motifs motif search transcription regulation 

Notes

Acknowledgments

I am grateful to Dr. Ferenc Marincs for critical reading of the manuscript and for his helpful suggestions.

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Copyright information

© Humana Press Inc. 2007

Authors and Affiliations

  • Endre Barta
    • 1
  1. 1.Agricultural Biotechnology Center, Bioinformatics GroupHungary

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