Lipid Rafts pp 73-84 | Cite as

Fluorescence Correlation Spectroscopy

  • Kirsten Bacia
  • Petra Schwille
Part of the Methods in Molecular Biology book series (MIMB, volume 398)

Abstract

Fluorescence correlation spectroscopy (FCS) is a technique that allows for an extremely sensitive determination of molecular diffusion properties, down to the level of single molecules. It thus provides an attractive alternative to FRAP, requiring much less laser power and lower concentrations of fluorophores. FCS has recently been applied on live cells, and in comparison on domain-forming model membrane systems, to systematically study the influence of cholesterol on local membrane structure by investigating the mobility of selected lipid probes. The findings demonstrate the ability of FCS to sensitively distinguish between different local lipid structures, and emphasize the value of model systems for understanding membrane dynamics in general.

Key Words

Confocal microscopy diffusion fluorescence fluctuations giant unilamellar vesicles liquid-disordered liquid-ordered single molecules 

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Copyright information

© Humana Press Inc., Totowa, NJ 2007

Authors and Affiliations

  • Kirsten Bacia
    • 1
  • Petra Schwille
    • 1
  1. 1.Institute of BiophysicsDresden University of TechnologyDresdenGermany

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