Volume 394 of the series Methods in Molecular Biology pp 133-175

Salmonella Phages and Prophages—Genomics and Practical Aspects

  • Andrew M. KropinskiAffiliated withHost and Pathogen Determinants, Laboratory for Foodborne Zoonoses, Public Health Agency of CanadaDepartment of Microbiology and Immunology, Queen’s University
  • , Alexander SulakvelidzeAffiliated withIntralytix, Inc.
  • , Paulina KonczyAffiliated withLaboratory for Foodborne Zoonoses, Public Health Agency of Canada
  • , Cornelius PoppeAffiliated withLaboratory for Foodborne Zoonoses, Public Health Agency of Canada

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Numerous bacteriophages specific to Salmonella have been isolated or identified as part of host genome sequencing projects. Phylogenetic analysis of the sequenced phages, based on related protein content using CoreGenes, reveals that these viruses fall into five groupings (P27-like, P2-like, lambdoid, P22-like, and T7-like) and three outliers (ɛ15, KS7, and Felix O1). The P27 group is only represented by ST64B; the P2 group contains Fels-2, SopEφ, and PSP3; the lambdoid Salmonella phages include Gifsy-1, Gifsy-2, and Fels-1. The P22-like viruses include ɛ34, ES18, P22, ST104, and ST64T. The only member of the T7-like group is SP6. The properties of each of these phages are discussed, along with their role as agents of genetic exchange and as therapeutic agents and their involvement in phage typing.

Key Words

Bacteriophage temperate lytic prophage genome analysis genetic map genome evolution P22-like phages T7-like phages lambdoid phages