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Tissue-Print Immunodetection of Transgene Products in Endosperm for High-Throughput Screening of Seeds

  • M. Paul Scott
Part of the Methods in Molecular Biology™ book series (MIMB, volume 526)

Summary

This method allows high-throughput qualitative screening to identify seeds containing a transgene product in endosperm tissue. It is particularly useful for determining genetic segregation ratios or identifying seeds to be advanced in a breeding program. Tissue printing is used to avoid time-consuming extraction steps. Antibody-based detection of the transgene product makes this method suitable to any transgene product for which a specific antibody is available. It is possible to screen thousands of seeds per week using this method.

Keywords

Seed Endosperm Antibody Protein 

Notes

Acknowledgments

The author wishes to thank Mrs. Merinda Struthers for technical assistance.

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  1. 1.USDA-ARSCorn Insects and Crop Genetics Research UnitAmesUSA

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