Protocol

Mass Spectrometry of Proteins and Peptides

Volume 492 of the series Methods In Molecular Biology pp 309-320

Proteomic Global Profiling for Cancer Biomarker Discovery

  • Vitor FacaAffiliated withFred Hutchinson Cancer Research Center
  • , Hong WangAffiliated withFred Hutchinson Cancer Research Center
  • , Samir HanashAffiliated withFred Hutchinson Cancer Research Center

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Summary

The ultimate goal of cancer molecular diagnostics is the development of simple tests to predict cancer risk, detect cancer early, classify tumors, and monitor response to therapy. Proteomics is well suited for these tasks. However, there are substantial challenges that need to be met to identify the most informative markers using proteomics. Approaches for in-depth quantitative proteomic analysis based on isotopic labeling and protein fractionation are presented in this chapter.

Key words

Biomarkers Proteomics LC-MS/MS Intact proteins Acrylamide Isotopic labeling Serum Plasma Multidimensional liquid chromatography