Molecular Motors

Volume 392 of the series Methods in Molecular Biology™ pp 1-22

Equilibrium Binding of Proteins to F-Actin

  • Joseph M. ChalovichAffiliated withDepartment of Biochemistry and Molecular Biology, Brody School of Medicine, East Carolina University

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This chapter reviews some of the many available methods for measuring the binding of myosin and other proteins to actin. Binding to actin has special considerations because actin is a long lattice and the binding site of many of its binding partners consists of multiple actin protomers. The analysis of binding to a lattice cannot be done by standard methods such as a Scatchard plot. Rational methods of analysis are described.

Key Words

Myosin S1 actin G-actin F-actin cooperativity kinetics binding binding analysis equilibrium association constant parking problem ATPase rate equations sedimentation ATP