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Raman-Assisted X-Ray Crystallography for the Analysis of Biomolecules

  • Dominique BourgeoisEmail author
  • Gergely Katona
  • Eve de Rosny
  • Philippe Carpentier
Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 544)

Summary

In this chapter, we describe Raman microspectrophotometry applied to crystals of biomolecules. Raman spectra collected in crystallo provide structural information highly complementary to X-ray diffraction, relate the crystalline state to the solution state, and allow the identification of ligand-bound or intermediate states of macromolecules. Nonresonant Raman spectroscopy is particularly suitable to the study of macromolecular crystals, and therefore applies to a wide range of noncolored crystalline proteins. Practical issues related to the investigation of crystals by Raman microspectrophotometry are reviewed, and the current limitations are highlighted.

Key words

In crystallo Raman spectroscopy Macromolecules Microspectrophotometers Crystallography Complementary methods Nonresonant Raman spectroscopy 

Notes

Acknowledgments

This work received financial support from the European Mole­cular Biology Organisation (EMBO), the European Synchrotron Radiation Facility (Grenoble, France), “Ministère de l’Enseignement et de la Recherche,” and the “Région Rhônes-Alpes” (France, CPER and CIBLE contracts). Contributions by Antoine Royant, Vincent Nivière, Jeremy Ohana, David Annequin, and Michel Belleil are acknowledged.

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Dominique Bourgeois
    • 1
    Email author
  • Gergely Katona
    • 1
  • Eve de Rosny
    • 1
  • Philippe Carpentier
    • 1
  1. 1.Dominique BourgeoisInstitut de Biologie Structurale Jean-Pierre EbelGrenoble CedexFrance

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