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Molecular Design of Performance Proteins With Repetitive Sequences

Recombinant Flagelliform Spider Silk as Basis for Biomaterials
  • Charlotte Vendrely
  • Christian Ackerschott
  • Lin Römer
  • Thomas Scheibel
Part of the Methods in Molecular Biology™ book series (MIMB, volume 474)

Summary

Most performance proteins responsible for the mechanical stability of cells and organisms reveal highly repetitive sequences. Mimicking such performance proteins is of high interest for the design of nanostructured biomaterials. In this article, flagelliform silk is exemplary introduced to describe a general principle for designing genes of repetitive performance proteins for recombinant expression in Escherichia coli . In the first step, repeating amino acid sequence motifs are reversely transcripted into DNA cassettes, which can in a second step be seamlessly ligated, yielding a designed gene. Recombinant expression thereof leads to proteins mimicking the natural ones. The recombinant proteins can be assembled into nanostructured materials in a controlled manner, allowing their use in several applications.

Key Words

Biomaterials recombinant production repetitive sequence spider silk proteins 

Notes

Acknowledgments

We thank members of the Fiberlab and Lasse Reefschläger for critical comments on the manuscript. This work was supported by grants from DFG (SCHE 603/4-2) and ARO (W911NF-06-1-0451).

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Copyright information

© Humana Press, a part of Springer Science + Business Media, LLC 2008

Authors and Affiliations

  • Charlotte Vendrely
    • 1
  • Christian Ackerschott
    • 1
  • Lin Römer
    • 2
  • Thomas Scheibel
    • 2
  1. 1.Department Chemie, Lehrstuhl BiotechnologieTUMGarchingGermany
  2. 2.Lehrstuhl BiomaterialienUniversität BayreuthBayreuthGermany

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