Measurement of Abscisic Acid and Gibberellins by Gas Chromatography/Mass Spectrometry

  • Masanori Okamoto
  • Atsushi Hanada
  • Yuji Kamiya
  • Shinjiro Yamaguchi
  • Eiji Nambara
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 495)

Abstract

Gas chromatography–mass spectrometry (GC-MS)-based analysis is an accurate and sensitive method to quantify plant hormones. This method is commonly used for analysis of low-molecular-weight compounds, such as abscisic acid (ABA), gibberellins (GAs), auxins, and brassinosteroids. Procedures are composed of four major steps: extraction, fractionation, derivatization, and detection. Here, we describe a protocol for quantification of ABA and GAs.

Key words

Abscisic acid gibberellins gas chromatography–mass spectrometry (GC-MS) internal standard derivatization 

References

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    Priest, D. M., Ambrose, S. J., Vaistij, F. E., Elias, L., Higgins, G. S., Ross, A. R., Abrams, S. R. and Bowles, D. J. (2006) Use of the glucosyltransferase UGT71B6 to disturb abscisic acid homeostasis in Arabidopsis thaliana. Plant J 46, 492–502.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Masanori Okamoto
    • 1
  • Atsushi Hanada
    • 1
  • Yuji Kamiya
    • 1
  • Shinjiro Yamaguchi
    • 1
  • Eiji Nambara
    • 1
  1. 1.Growth Regulation Research GroupRIKEN Plant Science CenterTsurumiJapan

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