Construction of Gene-Targeting Vectors by Recombineering
Recombineering is a technology that utilizes the efficient homologous recombination functions encoded by λ phage to manipulate DNA in Escherichia coli. Construction of knockout vectors has been greatly facilitated by recombineering as it allows one to choose any genomic region to manipulate. We describe here an efficient recombineering-based protocol for making mouse conditional knockout targeting vectors.
Key wordsConditional knockout recombineering vector gene targeting mouse E. coli
This work is supported by The Wellcome Trust.
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