Overview on Mouse Mutagenesis

  • Ralf Kühn
  • Wolfgang Wurst
Part of the Methods in Molecular Biology book series (MIMB, volume 530)


In this chapter we give an overview of mutagenesis methods in the mouse as they evolved over the last two decades, an outlook of ongoing and future developments and advice for choosing a mutagenesis strategy. Where appropriate, reference is given to relevant chapters of this book, key original articles and links of web-based resources for mouse mutagenesis.

Key words

Knockout mice conditional mutagenesis Cre/loxP RNAi knockdown ENU mutagenesis chimaeric mice 


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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Ralf Kühn
    • 1
    • 2
  • Wolfgang Wurst
    • 3
    • 4
  1. 1.Institute for Developmental Genetics, Helmholtz Zentrum München – German Research Center for Environmental HealthMunichGermany
  2. 2.Lehrstuhl für EntwicklungsgenetikTechnische Universität MünchenMunichGermany
  3. 3.Institute for Developmental Genetics, Helmholtz Zentrum München – German Research Center for Environmental Health & Max-Plank-Institute for PsychiatryMunichGermany
  4. 4.Lehrstuhl für EntwicklungsgenetikTechnische Universität MünchenMunichGermany

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