Fluorescent Protein Fusions for Protein Localization in Plants

  • John Runions
  • Chis Hawes
  • Smita Kurup
Part of the Methods in Molecular Biology™ book series (MIMB, volume 390)

Protein localization in living plant cells is commonly studied using fluorescent protein fusions. Stable transformation of plant cells requires the use of binary vectors, which are larger and not as amenable to genetic manipulation as animal cell transfection vectors. Binary vectors containing fluorescent protein fusion constructs are prepared using standard molecular biological techniques. Fusion genes as well as promoters and selection markers are stably incorporated into the plant cell genome via Agrobacterium-mediated transfer. Presented here are a series of protocols that detail binary vector construction, bacterial transformation, and a rapid transient assay technique that can be used to evaluate fusion protein fluorescence in leaves.

Key Words

Fluorescent protein green fluorescent protein binary vector Agrobacterium Nicotiana tobacco Arabidopsis


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Copyright information

© Humana Press Inc. 2007

Authors and Affiliations

  • John Runions
    • 1
  • Chis Hawes
    • 1
  • Smita Kurup
    • 2
  1. 1.School of Life SciencesOxford Brookes UniversityOxfordUK
  2. 2.CPI DivisionRothamsted ResearchHertfordshireUK

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