Baculovirus and Insect Cell Expression Protocols

Volume 388 of the series Methods in Molecular Biology™ pp 379-404

Evaluation of the Insecticidal Efficacy of Wild-Type and Recombinant Baculoviruses

  • Huarong LiAffiliated withDepartment of Entomology, Iowa State University
  • , Bryony C. BonningAffiliated withDepartment of Entomology, Iowa State University

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A considerable amount of work has been done during the last 20 yr to genetically enhance the efficacy of baculovirus insecticides. Following construction of a genetically altered baculovirus, laboratory bioassays are used to quantify various parameters of insecticidal activity such as the median lethal concentration (or dose) required to kill 50% of infected larvae (LC50 or LD50), median survival time of larvae infected at a fixed dose (ST50), and feeding damage incurred by infected larvae. In this chapter, protocols are described for a variety of bioassays and corresponding data analyses for assessment of the insecticidal activity or host range of baculovirus insecticides. Methods are also provided for baculovirus inoculation of larvae using a microapplicator for determining ST50 or for examining physiological effects.

Key Words

Recombinant baculovirus insecticidal efficacy host range droplet feeding diet plug bioassay feeding damage microapplicator LC50 LD50 ST50