Evaluation of the Insecticidal Efficacy of Wild-Type and Recombinant Baculoviruses

  • Huarong Li
  • Bryony C. Bonning
Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 388)

Abstract

A considerable amount of work has been done during the last 20 yr to genetically enhance the efficacy of baculovirus insecticides. Following construction of a genetically altered baculovirus, laboratory bioassays are used to quantify various parameters of insecticidal activity such as the median lethal concentration (or dose) required to kill 50% of infected larvae (LC50 or LD50), median survival time of larvae infected at a fixed dose (ST50), and feeding damage incurred by infected larvae. In this chapter, protocols are described for a variety of bioassays and corresponding data analyses for assessment of the insecticidal activity or host range of baculovirus insecticides. Methods are also provided for baculovirus inoculation of larvae using a microapplicator for determining ST50 or for examining physiological effects.

Key Words

Recombinant baculovirus insecticidal efficacy host range droplet feeding diet plug bioassay feeding damage microapplicator LC50 LD50 ST50 
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Copyright information

© Humana Press Inc., Totowa, NJ 2007

Authors and Affiliations

  • Huarong Li
    • 1
  • Bryony C. Bonning
    • 1
  1. 1.Department of EntomologyIowa State UniversityAmes

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