Gene Expression Analysis in Diabetes Research

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 560)

Summary

Global gene expression profiling through the use of microarray technology is among the most powerful molecular biological techniques available to diabetes researchers today. In this chapter, we outline how to appropriately perform a microarray experiment using pancreatic islets or total pancreas, based upon over a decade of experience in our laboratory. Through the utilization of careful experimental designs, large numbers of biological replicates, production of high-quality starting material, optimized protocols for hybridization, and sophisticated tools for data processing and statistical analysis, the full potential of high-quality expression profiling can be realized.

Key words

Microarray RNA extraction Gene expression profiling Diabetes Islet, β-Cell 

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  1. 1.Department of Genetics and Institute for Diabetes, Obesity and MetabolismUniversity of Pennsylvania School of MedicinePhiladelphiaUSA

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