Skeletal (“Mesenchymal”) Stem Cells for Tissue Engineering

  • Pamela Gehron Robey
  • Sergei A. Kuznetsov
  • Mara Riminucci
  • Paolo Bianco
Protocol
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 140)

Summary

Skeletal stem cells (SSCs, commonly referred to as “mesenchymal” stem cells) are found in the bone marrow stromal cell (BMSC) fraction of post-natal bone marrow. They can be isolated in culture as adherent, clonogenic cells endowed with the ability to grow and differentiate into multiple lineages, all of which correspond to tissues that are integral parts of the skeleton. The multipotency of SSCs is probed by in vivo transplantation assays. The ability of SSCs to generate a cell strain competent to form significant amounts of bone in vivo has led to the formulation of preclinical models of bone repair.

Key Words

Skeletal stem cells Mesenchymal stem cells Osteogenesis Bone repair Tissue engineering Bone Cartilage 
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Copyright information

© Humana Press Inc. 2007

Authors and Affiliations

  • Pamela Gehron Robey
    • 1
  • Sergei A. Kuznetsov
    • 1
  • Mara Riminucci
    • 2
  • Paolo Bianco
    • 3
  1. 1.Department of Health and Human Services, Craniofacial and Skeletal Diseases BranchNational Institute of Dental and Craniofacial Research, National Institutes of HealthBethesda
  2. 2.Department of Experimental MedicineUniversity of L’AquilaRomeItaly
  3. 3.Department of Experimental Medicine and Pathology, Department of Health and Human Services, Craniofacial and Skeletal Diseases BranchLa Sapienza University, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MDRomeItaly

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