• Harinder P. S. Makkar
  • P. Siddhuraju
  • Klaus Becker
Part of the Methods in Molecular Biology™ book series (MIMB, volume 393)


Saponins comprise a large family of structurally related compounds containing a steroid or triterpenoid aglycone (sapogenin) linked to one or more oligosaccharide moieties by glycosidic linkage (Fig. 1). The carbohydrate moiety consists of pentoses, hexoses, or uronic acids. The presence of both polar (sugar) and nonpolar (steroid or triterpene) groups provides saponins with strong surface-active properties that then are responsible for many of its adverse and beneficial effects. The primary biological effect of saponins is the interactions with cellular and membrane components. For example, saponins hemolyze red blood cells by nonspecific interactions with membrane proteins, phospholipids, and cholesterol of erythrocytes.

Key Words

Steroidal saponins triterpenoid saponins sapogenins hemolytic activity foaming hypocholesterolemic effects medicagenic acid sapogenin diosgenin vanillinperchloric acid reagent TLC plate fenugreek Quillaja Yucca 


  1. 1.
    Baccou, J. C., Lambert, F., and Sauvaire, Y. (1977) Spectrophotometric method for the determination of total steroidal sapogenin. Analyst 102, 458–465.PubMedCrossRefGoogle Scholar
  2. 2.
    Hiai, S., Oura, H., and Nakajima, T. (1976) Color reaction of some sapogenins and saponins with vanillin and sulphuric acid. Planta Medica 29, 116–122.PubMedCrossRefGoogle Scholar
  3. 3.
    Francis, G., Kerem, Z., Makkar, H. P. S., and Becker, K. (2002) The biological action of saponins in animal systems: a review. Br. J. Nutr. 88, 587–605.PubMedCrossRefGoogle Scholar
  4. 4.
    Burbano, C., Muzquiz, M., Ayet, G., Cuadrado, C., and Pedrosa, M. M. (1999) Evaluation of antinutritional factors of selected varieties of Phaseolus vulgaris. J. Sci. Food Agric. 79, 1468–1472.CrossRefGoogle Scholar
  5. 5.
    Siddhuraju, P., Becker, K., and Makkar, H. P. S. (2002) Chemical composition, protein fractionation, essential amino acid potential and antimetabolic constituents of an unconventional legume, Gila bean (Entada phaseoloides Merrill) seed kernel. J. Sci. Food Agric. 82, 192–202.CrossRefGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2007

Authors and Affiliations

  • Harinder P. S. Makkar
    • 1
  • P. Siddhuraju
    • 1
  • Klaus Becker
    • 1
  1. 1.Institute for Animal Production in the Tropics and SubtropicsUniversity of HohenheimStuttgartGermany

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