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Synthesis of Toll-Like Receptor-2 Targeting Lipopeptides as Self-Adjuvanting Vaccines

  • Brendon Y. Chua
  • Weiguang Zeng
  • David C. Jackson
Part of the Methods In Molecular Biology™ book series (MIMB, volume 494)

Summary

Effective Th1- and Th2-type immune responses that result in protective immunity against pathogens can be induced by self-adjuvanting lipopeptides containing the lipid moiety dipalmitoyl-S-glyceryl cysteine (Pam2Cys). The potent immunogenicity of these lipopeptides is due to their ability to activate dendritic cells by targeting and signaling through Toll-like receptor-2 (TLR-2). In addition, the simplicity and flexibility in their design as well as their ease of chemical definition and characterisation makes them highly attractive vaccine candidates for humans and animals. We describe in this chapter the techniques involved in the synthesis of an immunocontraceptive lipopeptide vaccine as well as the experimental assays carried out to evaluate its efficiency.

Keywords

Self-adjuvanting lipopeptide peptide synthesis ELISA antibody immunocontraception 

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Brendon Y. Chua
    • 1
  • Weiguang Zeng
    • 1
  • David C. Jackson
    • 1
  1. 1.Department of Microbiology and ImmunologyUniversity of MelbourneParkvilleAustralia

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