Phenotypic Analysis of B-Cells and Plasma Cells

  • Henrik E. Mei
  • Taketoshi Yoshida
  • Gwendolin Muehlinghaus
  • Falk Hiepe
  • Thomas Dörner
  • Andreas Radbruch
  • Bimba F. Hoyer
Part of the Methods in Molecular Medicine book series (MIMM, volume 136)

Abstract

B-cells and antibody-secreting plasma cells are key players in protective immunity, but also in autoimmune disease. To understand their various functions in the initiation and maintenance of autoimmune pathology, a detailed dissection of their functional diversity is mandatory. This requires a detailed phenotypic classification of the diversity of B-cells. Here, technologies of immunocytometry and ELISpot are described in detail, and their value for phenotypic characterization of cells of the B lineage, as well as for preparative cell sorting, to further characterize them functionally and on the molecular level are described.

Key Words

B-cells plasma cells flow cytometry intracellular Ig surface Ig secreted Ig ELISpot antigen specific memory B-cells naïve B-cells FACS autoimmunity cytometric secretion assay 

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Copyright information

© Humana Press Inc., Totowa, NJ 2007

Authors and Affiliations

  • Henrik E. Mei
    • 1
  • Taketoshi Yoshida
    • 1
  • Gwendolin Muehlinghaus
    • 1
  • Falk Hiepe
    • 2
  • Thomas Dörner
    • 3
  • Andreas Radbruch
    • 1
  • Bimba F. Hoyer
    • 2
  1. 1.German Rheumatism Research CentreBerlinGermany
  2. 2.Rheumatology and Clinical Immunology, CharitéClinic for Internal MedicineBerlinGermany
  3. 3.Institute of Transfusion Medicine, CharitéBerlinGermany

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