Hepatitis C pp 415-426

Part of the Methods in Molecular Biology™ book series (MIMB, volume 510)

Analysis of HCV-Specific T Cells by Flow Cytometry

  • Masaaki Shiina
  • Barbara Rehermann

Abstract

Flow cytometry has become an essential research tool because of the increase in the number of its {applications.} The development of an increasing number of monoclonal antibodies (mAbs) and fluorochromes, and of instruments capable of multicolor detection, allows the acquisition of a large amount of phenotypic and functional information in a single assay. In addition, flow-cytometry techniques have overcome critical problems of conventional assays, such as the use of radioactive reagents to assess proliferation and cytotoxicity of virus-specific T cells. Here, we provide both an overview of available techniques as well as standard protocols that have proven valuable in the assessment of HCV-specific T-cell responses.

Key Words

Flow cytometry FACS hepatitis C virus proliferation cytokines cytotoxic T cells 

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Masaaki Shiina
    • 1
  • Barbara Rehermann
    • 1
  1. 1.Immunology Section, Liver Diseases Branch, National Institute of Diabetes and Digestive and Kidney diseasesNational Institutes of HealthBethesdaUSA

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