Hepatitis C pp 403-413

Part of the Methods in Molecular Biology™ book series (MIMB, volume 510)

Determination of HCV-Specific T-Cell Activity

  • Joo Chun Yoon
  • Barbara Rehermann

Abstract

The magnitude and breadth of T-cell responses against HCV are associated with the outcome of HCV infection. Parameters of HCV-specific T-cell responses that are frequently assessed in clinical immunological studies include proliferation of T cells in response to HCV antigens, frequency of HCV-specific T cells secreting cytokines, and changes in antigen specificity during the course of HCV infection. Common techniques for assessing these parameters such as 3H-thymidine incorporation assay, cytokine ELISpot assay, and strategies for epitope mapping and identification of minimal optimal epitopes are outlined, and detailed protocols are described.

Key Words

Hepatitis C virus T cell proliferation assay ELISpot assay epitope mapping 

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Joo Chun Yoon
    • 1
  • Barbara Rehermann
    • 1
  1. 1.Immunology Section, Liver Diseases Branch, National Institute of Diabetes and Digestive and Kidney diseasesNational Institutes of HealthBethesdaUSA

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