Hepatitis C pp 15-23 | Cite as

Simultaneous Detection of Anti-HCV Antibody and HCV Core Antigen

  • Filippo Ansaldi
  • Giancarlo Icardi
Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 510)

Abstract

HCV infection is usually diagnosed by means of an enzyme immune assay for the detection of antibody against HCV. The window period between infection and seroconversion remains a dramatic problem in the transfusional and diagnostic setting. In this chapter, we report (i) procedures for assays using two different approaches designed to reduce the window period and (ii) performance in terms of specificity and sensitivity in the detection of both antibody and antigen, and we compare their efficacy with that of commercial assays.

Key Words

HCV diagnosis window period antigen antibody combination assay immunoassay chemiluminescence 
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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Filippo Ansaldi
    • 1
  • Giancarlo Icardi
    • 1
  1. 1.Department of Health SciencesUniversity of GenoaGenoaItaly

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