Mitochondria pp 421-430 | Cite as

Assessing Mitochondrial Potential, Calcium, and Redox State in Isolated Mammalian Cells Using Confocal Microscopy

  • Sean M. Davidson
  • Derek Yellon
  • Michael R. Duchen
Part of the Methods in Molecular Biology™ book series (MIMB, volume 372)


Mitochondria play a vital role in the regulation of intracellular calcium dynamics. Fluorescent dyes can be used to provide a direct measurement of the redox state, mitochondrial membrane potential, and mitochondrial calcium content. The simplicity of this approach lends itself to high-throughput assays and time-resolved analyses; however, care must be taken to avoid artifactual results. We outline general methods using confocal microscopy for analysis of the redox state, mitochondrial membrane potential, and mitochondrial calcium content in adult cardiomyocytes. We demonstrate how these parameters can be analyzed in parallel using the emission spectra “fingerprinting” method even when emission spectra overlap.

Key Words

Calcium cardiomyocytes confocal microscopy membrane potential NADH redox state 


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Copyright information

© Humana Press Inc., Totowa, NJ 2007

Authors and Affiliations

  • Sean M. Davidson
    • 1
  • Derek Yellon
    • 1
  • Michael R. Duchen
    • 2
  1. 1.The Hatter Institute and Centre for CardiologyUniversity College LondonLondonUK
  2. 2.Department of PhysiologyUniversity College LondonLondonUK

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