In Vitro Cyclooxygenase Activity Assay in Tissue Homogenates

  • Samir S. Ayoub
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 644)

Abstract

The use of in vitro cyclooxygenase (COX) activity assays has been particularly useful for comparing the effect of different drugs on COX activity in different tissues. In addition, this assay is relatively quick, cheap, and a large number of samples can be tested at the same time. However, one limitation of this assay is the fact that it does not discriminate between the activities of different COX isoforms.

Key words

COX activity Adrenaline Glutathione Arachidonic acid 

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Copyright information

© Springer Science+Busidness Media, LLC 2010

Authors and Affiliations

  • Samir S. Ayoub
    • 1
  1. 1.Centre for Biochemical Pharmacology, Experimental Pathology GroupSt. Bart’s and the London School of Medicine and Dentistry, William Harvey Research Institute, Queen Mary University of LondonLondonUK

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