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Cryopreservation of Red Blood Cells and Platelets

  • Andreas Sputtek
Part of the Methods in Molecular Biology™ book series (MIMB, volume 368)

Abstract

Blood cells can be regarded as a classical field of application of low-temperature biology. Cryopreservation methods have been developed for different categories of blood cells namely red blood cells (RBCs) (erythrocytes), platelets (thrombocytes), mononuclear cells (i.e., lymphocytes, monocytes), and hematopoietic progenitor cells. This chapter outlines the four most commonly applied techniques for RBCs and two for platelets.

Key Words

Cryopreservation red blood cells platelets dimethyl sulfoxide hydroxyethyl starch 

References

  1. 1.
    Sputtek, A. and Körber, C. (1991) Cryopreservation of red blood cells, platelets, lymphocytes, and stem cells. In: Clinical Applications of Cryobiology, (Fuller, B. J. and Grout, B. W. W., eds.), CRC Press, Boca Raton, FL, pp. 95–147.Google Scholar
  2. 2.
    Sputtek, A. and Sputtek, R. (2004) Cryopreservation in transfusion medicine and hematology. In: Life in the Frozen State, (Fuller, B. J., Benson E. E., and Lane, N. eds.), CRC Press, Boca Raton, FL, pp. 483–504.CrossRefGoogle Scholar
  3. 3.
    Meryman, H. T. and Hornblower, M. (1972) A method for freezing and washing red blood cells using a high glycerol concentration. Transfusion 12, 145–156.Google Scholar
  4. 4.
    Brecher, M. E. (ed.) (2003) Red cell cryopreservation using high-concentration glycerol—Meryman method, in Technical Manual, 14th ed., AABB, Bethesda, MD, pp. 741–743.Google Scholar
  5. 5.
    Rowe, A. W., Eyster, E., and Kellner, A. (1968) Liquid nitrogen preservation of red blood cells for transfusion: a low glycerol-rapid freeze procedure. Cryobiology 5, 119–128.CrossRefGoogle Scholar
  6. 6.
    Rowe, A. W. (1973) Preservation of blood by the low glycerol-rapid freeze process. In: Red Cell Freezing, American Association of Blood Banks (ed.), Washington, DC, pp. 55–71.Google Scholar
  7. 7.
    Sputtek, A. and Rau, G. (1992) Cryopreservation of human erythrocytes with hydroxyethyl starch (HES)-Part 1: The procedure. Infusionsther Transfusionsmed 19, 269–275.Google Scholar
  8. 8.
    Sputtek, A., Singbartl, G., Langer, R., Schleinzer, W., Henrich, H. A., and Kühnl, P. (1995) Cryopreservation of red blood cells with the non-penetrating cryoprotectant hydroxyethyl starch. CryoLetters 16, 283–288.Google Scholar
  9. 9.
    Thomas, M. J. G., Perry, E. S., Nash, S. H., and Bell, S. H. (1996) A method for the cryopreservation of red blood cells using hydroxyethyl starch a cryoprotectant. Transfus. Sci. 17, 385–396.CrossRefGoogle Scholar
  10. 10.
    Bell, S. M. and Thomas, M. J. G. (1995) Cryopreservation of human red blood cells. In: Cryopreservation and Freeze-Drying Protocols, (Day, J. G. and McLellan, M. R., eds.), Humana Press, Totowa, NJ, pp. 235–250.Google Scholar
  11. 11.
    Schiffer, C. A., Aisner, J., and Dutcher, J. P. (1985) Platelet cryopreservation using dimethyl sulfoxide. Ann. NY Acad. Sci. 459, 161–169.CrossRefGoogle Scholar
  12. 12.
    Walker, R. H. (ed.) (1993) Method 10.16. Platelet cryopreservation using 5% DMSO—Schiffer method, in Technical Manual, 11th ed., AABB, Bethesda, MD, pp. 733–737.Google Scholar
  13. 13.
    Choudhury, C. and Gunstone, M. J. (1978) Freeze preservation of platelets using hydroxyethyl starch (HES): a preliminary report. Cryobiology 15, 493–501.CrossRefGoogle Scholar
  14. 14.
    Huggins, C. E. (1963) Preservation of blood by freezing with dimethyl sulfoxide and its removal by dilution and erythrocyte agglomeration. Vox Sang. 8, 99–100.Google Scholar
  15. 15.
    Krijnen, H. W., Kuivenhoven, A. C. J., and De Wit, J. J. F. M. (1968) The preservation of blood cells in the frozen state. Experiences and current methods in the Netherlands. Cryobiology 5, 136–143.CrossRefGoogle Scholar
  16. 16.
    Rinfret, A. P. (1963) Some aspects of preservation of blood by rapid freeze-thaw procedures. Fed. Proc. 22, 94–101.Google Scholar
  17. 17.
    Knorpp, C. T., Merchant, W. R., Gikas, P. W., Spencer, H. H., and Thompson, N. W. (1967) Hydroxyethyl starch: extracellular cryophylactic agent for erythrocytes, Science 57, 1312–1313.CrossRefGoogle Scholar
  18. 18.
    Horn, P., Sputtek, A., Standl, Th., Rudolf, B., Kühnl, P., and Schulte am Esch, J. (1997) Transfusion of autologous, hydroxyethyl starch cryopreserved red blood cells in patients. Anaesth. Analg. 85, 739–745.CrossRefGoogle Scholar
  19. 19.
    Robson, D. C. (1970) Rapid freezing and thawing techniques using extra-cellular agents. In: Modern Problems of Blood Preservation, (Spielmann, W. and Seidl, S., eds.), Fischer, Stuttgart, Germany, pp. 204–208.Google Scholar
  20. 20.
    Klein, E., Toch, R., Farber, S., Freeman, G., and Fiorentino, R. (1956) Hemostasis in thrombocytopenic bleeding following infusion of stored, frozen platelets. Blood 11, 693–698.Google Scholar
  21. 21.
    Dayian, G. and Rowe, A. W. (1976) Cryopreservation of human platelets for transfusion. A glycerol-glucose, moderate rate cooling procedure. Cryobiology 13, 1–8.CrossRefGoogle Scholar
  22. 22.
    Ashwood-Smith, M. J. (1961) Preservation of mouse marrow at −79°C with dimethyl sulphoxide. Nature 190, 1204–1205.CrossRefGoogle Scholar
  23. 23.
    Stiff, P. J. (1995) Cryopreservation of hematopoietic stem cells. In: Marrow and Stem Cell Processing for Transplantation, (Lasky, L. C. and Warkentin P. I., eds.), American Association of Blood Banks, Bethesda, MD, pp. 69–82.Google Scholar
  24. 24.
    Bank, H. (1980) Granulocyte preservation circa 1980. Cryobiology 17, 187–197.CrossRefGoogle Scholar
  25. 25.
    Knight, S. C. (1980) Preservation of leucocytes. In: Low Temperature Preservation in Medicine and Biology, (Ashwood-Smith, M. J. and Farrant, J., eds.), Pitman Medical, Tunbridge Wells, UK, pp. 121–138.Google Scholar
  26. 26.
    Takahashi, T., Hammett, M., and Cho, M. S. (1985) Multifaceted freezing injury in human polymorphonuclear cells at high subfreezing temperatures. Cryobiology 22, 215–236.CrossRefGoogle Scholar
  27. 27.
    Brecher, M. E. (ed.) (2003) Technical Manual, 14th ed., AABB, Bethesda, MD, pp. 738–739.Google Scholar
  28. 28.
    Brecher, M. E. (ed.) (2003) Technical Manual, 14th ed., AABB, Bethesda, MD, pp. 748–750.Google Scholar
  29. 29.
    Sputtek, A., Brohm, A., Classen, I., et al. (1987) Cryopreservation of human platelets with hydroxyethyl starch in a one-step procedure. CryoLetters 8, 216–229.Google Scholar
  30. 30.
    Sputtek, A., Barthel, B., and Rau, G. (1993) Cryopreservation of human platelets: An in vitro comparison of a 4% HES method with the standard 5% DMSO method. Cryobiology 30, 656–657.Google Scholar
  31. 31.
    Pegg, D. E., Wusteman, M. C., and Boylan, S. (1997) Fractures in elastic arteries. Cryobiology 34, 183–192.CrossRefGoogle Scholar
  32. 32.
    Chen, T., Bhowmick, S., Sputtek, A., Fowler, A., and Toner, M. (2002) The glass transition temperature of mixtures of trehalose and hydroxyethyl starch. Cryobiology 44, 301–306.CrossRefGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2007

Authors and Affiliations

  • Andreas Sputtek
    • 1
  1. 1.Universitätsklinikum Hamburg-Eppendorf, Institut für TransfusionsmedizinHamburgGermany

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