Using RNA Interference to Knock Down the Adhesion Protein TES

  • Elen Griffith
Part of the Methods in Molecular Biology™ book series (MIMB, volume 370)


RNA interference (RNAi) is a specific and efficient method to knock down protein levels using small interfering RNAs (siRNAs), which target mRNA degradation. RNAi can be used in mammalian cell culture systems to target any protein of interest, and several studies have used this method to knock down adhesion proteins. We used siRNAs to knock down the levels of TES, a focal adhesion protein, in HeLa cells. We demonstrated knockdown of both TES mRNA and TES protein. Although total knockdown of TES was not achieved, the observed reduction in TES protein was sufficient to result in a cellular phenotype of reduced actin stress fibers.

Key Words

TES RNAi focal adhesion actin stress fibers HeLa 


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Copyright information

© Humana Press Inc., Totowa, NJ 2007

Authors and Affiliations

  • Elen Griffith
    • 1
  1. 1.MRC Human Genetics UnitWestern General HospitalEdinburghUK

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