Conjugation of Colloidal Gold to Proteins

  • Constance Oliver
Part of the Methods in Molecular Biology book series (MIMB, volume 588)


The ability to conjugate proteins to colloidal gold sols provides a wide variety of probes for electron microscopy. Antibodies, protein A, protein G, lectins, enzymes, toxins, and other proteins have all been conjugated to colloidal gold. The nature of the interaction between the colloidal gold and the protein is poorly understood. Proteins are conjugated to gold sols by adjusting the pH of the gold sol to approximately 0.5 pH unit higher than the pI of the protein being conjugated. This chapter gives a general method for conjugating proteins to colloidal gold as well as more specific methods for conjugating antibodies and protein A to colloidal gold.

Key words

Antibody Protein A Protein G Conjugation Colloidal gold 


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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.Department of Cell and Molecular Biology and Pathogenic Bioagents, Faculdade de Medicina de Ribeirão PretoUniversity of São PauloRibeirão PretoBrazil

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