Monoclonal Antibodies pp 15-31

Part of the Methods in Molecular Biology book series (MIMB, volume 378)

Recombinant Monoclonal Antibodies

  • Mariel Donzeau
  • Achim Knappik

Abstract

Recombinant antibody technology is a rapidly evolving field that enables the study and improvement of antibody properties by means of genetic engineering. Moreover, the functional expression of antibody fragments in Escherichia coli has formed the basis for antibody library generation and selection, a powerful method to produce human antibodies for therapy. Because in vitro-generated antibodies offer various advantages over traditionally produced monoclonal antibodies, such molecules are now increasingly used for standard immunological assays. This chapter will give a short review on how recombinant antibodies are generally be produced and engineered, and how typical immunoassays are performed.

Key Words

Recombinant antibodies antibody engineering Fab scFv antibody libraries phage display 

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Copyright information

© Humana Press Inc., Totowa, NJ 2007

Authors and Affiliations

  • Mariel Donzeau
    • 1
  • Achim Knappik
    • 2
  1. 1.Department of ResearchMorphoSys AGMartinsriedGermany
  2. 2.Global Head of ResearchMorphoSys AGMartinsriedGermany

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