Protocol

Monoclonal Antibodies

Volume 378 of the series Methods in Molecular Biology pp 15-31

Recombinant Monoclonal Antibodies

  • Mariel DonzeauAffiliated withDepartment of Research, MorphoSys AG
  • , Achim KnappikAffiliated withGlobal Head of Research, MorphoSys AG

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Abstract

Recombinant antibody technology is a rapidly evolving field that enables the study and improvement of antibody properties by means of genetic engineering. Moreover, the functional expression of antibody fragments in Escherichia coli has formed the basis for antibody library generation and selection, a powerful method to produce human antibodies for therapy. Because in vitro-generated antibodies offer various advantages over traditionally produced monoclonal antibodies, such molecules are now increasingly used for standard immunological assays. This chapter will give a short review on how recombinant antibodies are generally be produced and engineered, and how typical immunoassays are performed.

Key Words

Recombinant antibodies antibody engineering Fab scFv antibody libraries phage display