Volume 382 of the series Methods in Molecular Biology pp 287-312

μParaflo™ Biochip for Nucleic Acid and Protein Analysis

  • Qi ZhuAffiliated withDepartment of Biology and Biochemistry, University of Houston
  • , Ailing HongAffiliated withAtactic Technologies Inc.
  • , Nijing ShengAffiliated withAtactic Technologies Inc.
  • , Xiaolin ZhangAffiliated withAtactic Technologies Inc.
  • , Anna MatejkoAffiliated withAtactic Technologies Inc.
  • , Kyu-Yeon JunAffiliated withDepartment of Biology and Biochemistry, University of Houston
  • , Onnop SrivannavitAffiliated withAtactic Technologies Inc.
  • , Erdogan GulariAffiliated withDepartment of Chemical Engineering, University of Michigan
  • , Xiaolian GaoAffiliated withDepartment of Biology and Biochemistry, University of Houston
    • , Xiaochuan ZhouAffiliated withAtactic Technologies Inc.

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We describe in this chapter the use of oligonucleotide or peptide microarrays (arrays) based on microfluidic chips. Specifically, three major applications are presented: (1) microRNA/small RNA detection using a microRNA detection chip, (2) protein binding and function analysis using epitope, kinase substrate, or phosphopeptide chips, and (3) protein-binding analysis using oligonucleotide chips. These diverse categories of customizable arrays are based on the same biochip platform featuring a significant amount of flexibility in the sequence design to suit a wide range of research needs. The protocols of the array applications play a critical role in obtaining high quality and reliable results. Given the comprehensive and complex nature of the array experiments, the details presented in this chapter is intended merely as a useful information source of reference or a starting point for many researchers who are interested in genomeor proteome-scale studies of proteins and nucleic acids and their interactions.

Key Words

Aptamer microarray digital photolithography epitope-antibody profiling epitope screening kinase profiling assay microfluidic biochip micro-RNA detection oligoncleotide microarray peptide microarray microfluidics parallel synthesis phosphopeptide-protein binding photogenerated acid picoarray protein-binding