Cryoelectron Microscopy of Icosahedral Virus Particles

  • Wen Jiang
  • Wah Chiu
Part of the Methods in Molecular Biology™ book series (MIMB, volume 369)

Abstract

With the rapid progresses in both instrumentation and computing, it is increasingly straightforward and routine to determine the structures of icosahedral viruses to subnanometer resolutions (6–10 Å) by cryoelectron microscopy and image reconstruction. In this resolution range, secondary structure elements of protein subunits can be clearly discerned. Combining the three-dimensional density map and bioinformatics of the protein components, the folds of the virus capsid shell proteins can be derived. This chapter will describe the experimental and computational procedures that lead to subnanometer resolution structural determinations of icosahedral virus particles. In addition, we will describe how to extract useful structural information from the three-dimensional maps.

Key Words

Cryo-EM cryoelectron microscopy icosahedral virus 3D reconstruction subnanometer resolution secondary structure elements structural fitting 

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Copyright information

© Humana Press Inc. 2007

Authors and Affiliations

  • Wen Jiang
    • 1
  • Wah Chiu
    • 2
    • 3
  1. 1.Department of Biological SciencesPurdue UniversityWest Lafayette
  2. 2.National Center For Macromolecular ImagingBaylor College of MedicineHouston
  3. 3.Verna and Marrs Mclean Department of Biochemistry and Molecular BiologyBaylor College of MedicineHouston

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