DNA Sequence Polymorphism Analysis Using DnaSP

  • Julio Rozas
Part of the Methods in Molecular Biology book series (MIMB, volume 537)


The analysis of DNA sequence polymorphisms and SNPs (single nucleotide polymorphisms) can provide insights into the evolutionary forces acting on populations and species. Available population-genetic methods, and particularly those based on the coalescent theory, have become the primary framework to analyze such DNA polymorphism data. Here, I explain some essential analytical methods for interpreting DNA polymorphism data and also describe the basic functionalities of the DnaSP software. DnaSP is a multi-propose program that allows conducting exhaustive DNA polymorphism analysis using a graphical user-friendly interface.

Key words

Molecular population genetics software DNA sequence polymorphisms SNPs, Neutrality tests Coalescent methods DnaSP 



We thank Sergios-Orestis Kolokotronis for his helpful comments and suggestions on the manuscript. This work was funded by grant BFU2007-62927 from the Dirección General de Investigación Científica y Técnica (Spain), and by grant 2005SGR00166 from Comissió Interdepartamental de Recerca i Innovació Tecnològica de Catalunya (Spain).


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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Julio Rozas
    • 1
  1. 1.Departament de Genètica, Facultat de BiologiaUniversitat de BarcelonaBarcelonaSpain

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