RNA pp 205-217 | Cite as

Validation of RNAi by Real Time PCR

Part of the Methods in Molecular Biology book series (MIMB, volume 703)


Real time PCR is the analytic tool of choice for quantification of gene expression, while RNAi is concerned with downregulation of gene expression. Together, they constitute a powerful approach in any loss of function studies of selective genes. We illustrate here the use of real time PCR to verify luciferase mRNA silencing.

Key words

RNA isolation reverse transcription real time PCR LightCycler luciferase shRNA RNAi 


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.The Bartholin Institute, Copenhagen University HospitalCopenhagenDenmark
  2. 2.Cellular and Metabolic Research SectionBiomedical Institute, University of CopenhagenCopenhagenDenmark

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