Explant Cultures of White Adipose Tissue

  • Sébastien Thalmann
  • Cristiana E. Juge-Aubry
  • Christoph A. Meier
Part of the Methods in Molecular Biology™ book series (MIMB, volume 456)


Obesity is characterized by increased adiposity of visceral and subcutaneous depots as well as other organs, including the vasculature. These fat depots secrete various hormone-like proteins implicated in metabolic homeostasis (e.g., adiponectin, resistin), the central control of appetite (e.g., leptin) and the increased production of cytokines. These molecules act either in a paracrine or endocrine manner, contributing to the metabolic and cardiovascular complications of obesity. Explant cultures of white adipose tissue are an important step in analyzing the secretory mechanisms of adipose tissue by preserving the physiological in vivo cross-talk between the various types of cells.

Key words

Obesity white adipose tissue tissue culture cytokines chemokines inflammation. 


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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Sébastien Thalmann
    • 1
  • Cristiana E. Juge-Aubry
    • 1
  • Christoph A. Meier
    • 2
    • 3
  1. 1.University of Geneva Medical SchoolGenevaSwitzerland
  2. 2.University of Geneva Medical SchoolGenevaSwitzerland
  3. 3.Department of Internal MedicineTriemli HospitalZürichSwitzerland

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