Isolation of Mycobacterium Species Genomic DNA

  • John T. Belisle
  • Spencer B. Mahaffey
  • Preston J. Hill
Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 465)

Abstract

A myriad of methods has been reported for the isolation of genomic DNA from Mycobacterium spp.; some methods use mechanical disruption of the bacterial cells, whereas others use some form of chemical or enzymatic lysis. Regardless of the approach, the end points remain efficient breaking of the complex mycobacterial cell wall and release of high-quality DNA that is suitable for manipulation and analyses by molecular genetic techniques. This chapter providers detailed methods for the large and small isolation of mycobacterial genomic DNA.

Keywords

Cell lysis DNA isolation genomic DNA Mycobacterium 
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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • John T. Belisle
    • 1
  • Spencer B. Mahaffey
    • 2
  • Preston J. Hill
    • 2
  1. 1.Mycobacteria Research Laboratories, Department of Microbiology, Immunology, and PathologyColorado State UniversityFort CollinsUSA
  2. 2.Department of Microbiology, Immunology, and PathologyColorado State UniversityFort CollinsUSA

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