Phagocytosis of Candida albicans by RNAi-Treated Drosophila S2 Cells

  • Shannon L. Stroschein-Stevenson
  • Edan Foley
  • Patrick H. O’Farrell
  • Alexander D. Johnson
Part of the Methods in Molecular Biology book series (MIMB, volume 470)


Phagocytosis is a highly conserved aspect of innate immunity. Drosophila melanogaster has an innate immune system with many similarities to that of mammals and has been used to successfully model many aspects of innate immunity. The recent availability of Ribo Nucleic Acid interference (RNAi) libraries for Drosophila has made it possible to efficiently screen for genes important in aspects of innate immunity. We have screened an RNAi library representing 7216 fly genes conserved among metazoans to identify proteins required for the phagocytosis of the human fungal pathogen Candida albicans.

Key words

Candida albicans Drosophila S2 cells cell culture RNAi double-stranded RNA phagocytosis immunofluorescence 


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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Shannon L. Stroschein-Stevenson
    • 1
  • Edan Foley
    • 2
  • Patrick H. O’Farrell
    • 3
  • Alexander D. Johnson
    • 4
  1. 1.Department of Microbiology and ImmunologyUniversity of California, San FranciscoSan FranciscoUSA
  2. 2.Department of Medical Microbiology and ImmunologyUniversity of AlbertaEdmontonCanada
  3. 3.Department of Biochemistry and BiophysicsUniversity of California, San FranciscoSan FranciscoUSA
  4. 4.Department of Microbiology and Immunology, and Department of Biochemistry and BiophysicsUniversity of California, San FranciscoSan FranciscoUSA

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